Journal: EBioMedicine

Article Title: Quantification, epitope mapping and genotype cross-reactivity of hepatitis B preS-specific antibodies in subjects vaccinated with different dosage regimens of BM32

doi: 10.1016/j.ebiom.2020.102953

Figure Lengend Snippet: Comparison of preS-specific isotype and IgG subclasses responses in the four groups of patients. Shown are optical density (OD) values ( y -axes) corresponding to preS-specific IgG, IgA, IgM, IgE and IgG subclass (IgG 1 –IgG 4 ) levels in patients immunized with 3, 4 or 5 injections of BM32 or with placebo (see inlay) at baseline (V3), after one (V8) or four (V11) months treatment ( x -axes). Medians (horizontal bars) and significant differences are indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The detection was performed using either biotin-labeled mouse anti-human IgG 1 (BD, Cat. 555869) or biotin-labeled mouse anti-human IgG 4 (BD, Cat. 555882) in dilution 1:1000 mixed with HRP-conjugated streptavidin (BD, Cat. 554066) in dilution 1:2500 and incubated overnight at 4 °C.

Techniques:

Journal: EBioMedicine

Article Title: Quantification, epitope mapping and genotype cross-reactivity of hepatitis B preS-specific antibodies in subjects vaccinated with different dosage regimens of BM32

doi: 10.1016/j.ebiom.2020.102953

Figure Lengend Snippet: Quantification of IgG 1 and IgG 4 subclass responses in patients treated with placebo or different numbers of BM32 injections. Shown are absolute increases of serum concentrations of IgG 1 and IgG 4 ( y -axes: μg/ml) specific (a) for preS, (b) for peptide A, (c) for peptide B and (d) for peptide C from baseline (V3) to visits 8 and 11 ( x -axes) inpatients immunized with placebo, 3, 4 or 5 injections of BM32. Medians (horizontal bars) and significant differences are indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The detection was performed using either biotin-labeled mouse anti-human IgG 1 (BD, Cat. 555869) or biotin-labeled mouse anti-human IgG 4 (BD, Cat. 555882) in dilution 1:1000 mixed with HRP-conjugated streptavidin (BD, Cat. 554066) in dilution 1:2500 and incubated overnight at 4 °C.

Techniques:

Journal: EBioMedicine

Article Title: Quantification, epitope mapping and genotype cross-reactivity of hepatitis B preS-specific antibodies in subjects vaccinated with different dosage regimens of BM32

doi: 10.1016/j.ebiom.2020.102953

Figure Lengend Snippet: Kinetics and fine specificities of anti-preS antibody responses in the individual subjects of the placebo group, and the groups having received 3, 4 or 5 injections. The heat maps depict the log 10 of the serum concentrations of preS- or peptide-specific (a) IgG 1 and (b) IgG 4 . Missing values are indicated in gray.

Article Snippet: The detection was performed using either biotin-labeled mouse anti-human IgG 1 (BD, Cat. 555869) or biotin-labeled mouse anti-human IgG 4 (BD, Cat. 555882) in dilution 1:1000 mixed with HRP-conjugated streptavidin (BD, Cat. 554066) in dilution 1:2500 and incubated overnight at 4 °C.

Techniques: